Composite

Part:BBa_K3782022:Design

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-20)


cspA-RBS-TEE-His-FfIBP-Term


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 533
    Illegal PstI site found at 614
    Illegal PstI site found at 794
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 533
    Illegal PstI site found at 614
    Illegal PstI site found at 794
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 533
    Illegal PstI site found at 614
    Illegal PstI site found at 794
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 533
    Illegal PstI site found at 614
    Illegal PstI site found at 794
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The signal peptide sequence (1-23) was excluded. The sequence was codon optimized for optimal expression in Escherichia coli.


Source

The source of FfIBP is the Gram-negative Antarctic bacterium Flavobacterium frigoris PS1. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/JQ712369 The part was synthesized with a 6x His tag.

References